期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1767, 期 6, 页码 847-853出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2007.01.010
关键词
thylakoid membrane; light-harvesting complex; circular dichroism; linear dichroism; time-resolved fluorescence
Aggregates and solubilized trimers of LHCII were characterized by circular dichroism (CD), linear dichroism and time-resolved fluorescence spectroscopy and compared with thylakoid membranes in order to evaluate the native state of LHCII in vivo. It was found that the CD spectra of lamellar aggregates closely resemble those of unstacked thylakoid membranes whereas the spectra of trimers solubilized in n-dodecyl-beta,D-maltoside, n-octyl-beta,D-glucopyranoside, or Triton X-100 were drastically different in the Soret region. Thylakoid membranes or LHCII aggregates solubilized with detergent exhibited CD spectra similar to the isolated trimers. Solubilization of LHCII was accompanied by profound changes in the linear dichroism and increase in fluorescence lifetime. These data support the notion that lamellar aggregates of LHCII retain the native organization of LHCII in the thylakoid membranes. The results indicate that the supramolecular organization of LHCII, most likely due to specific trimer-trimer contacts, has significant impact on the pigment interactions in the complexes. (c) 2007 Elsevier B.V All rights reserved.
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