期刊
GENETICS
卷 176, 期 2, 页码 913-925出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.106.066167
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In the unicellular algae Chlamydomonas reinhardtii, the plus and minus mating types are controlled by a Complex locus, MT, where the dominant MID gene in the MT- locus has been shown to be necessary for expression of minus-specific gamete-specific genes in response to nitrogen depletion. We report studies on MID expression patterns during gametogencsis and on a second gene unique to the MT- locus, MTD1 Vegetative cells express basal levels of MID. An early activation of MID transcription after nitrogen re-moval, and its sequence similarity to plant. RWP-RK proteins involved in nitrogen-responsive processes, Suggest that Mid conformation/activity may be nitrogen sensitive. A second stage of MID upregulation correlates with the acquisition of mating ability in minus gametes. Kuockdown of MTD1 by RNAi in minus strains results in a failure to differentiate into gametes of either mating type after nitrogen deprivation. We propose that intermediate Mid levels are sufficient to activate MTD1 transcription and to repress plus gamete-specific genes and that MTD1 expression in turn allows the threshold-level MID expression needed to turn on minus gamete-specific genes. We further propose that an MTD1-equivalent system, utilizing at least one gene product encoded in the MT+ locus, is operant during plus gametogenesis.
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