期刊
CELL CALCIUM
卷 41, 期 6, 页码 547-558出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2006.10.004
关键词
SNARE microdomains; calcium channels; exocytosis; bovine chromaffin cells; adrenomedullary slices
类别
资金
- Medical Research Council [MC_U105178791] Funding Source: Medline
- Medical Research Council [MC_U105178791] Funding Source: researchfish
- MRC [MC_U105178791] Funding Source: UKRI
Regulated exocytosis involves calcium-dependent fusion of secretory vesicles with the plasma membrane with three SNARE proteins playing a central role: the vesicular synaptobrevin and the plasma membrane syntaxin 1 and SNAP-25. Cultured bovine chromaffin cells possess defined plasma membrane microdomains that are specifically enriched in both syntaxin 1 and SNAP-25. We now show that in both isolated cells and adrenal medulla slices these target SNARE (t-SNARE) patches quantitatively coincide with single vesicle secretory spots as detected by exposure of the intravesicular dopamine P-hydroxylase onto the plasmalemma. During exocytosis, neither area nor density of the syntaxin 1/SNAP-25 microdomains changes on the plasma membrane of both preparations confirming that preexisting clusters act as the sites for vesicle fusion. Our analysis reveals a high level of colocalization of L, N and P/Q type calcium channel clusters with SNAREs in adrenal slices; this close association is altered in individual cultured cells. Therefore, microdomains carrying syntaxin I /SNAP-25 and different types of calcium channels act as the sites for physiological granule fusion in '' in situ '' chromaffin cells. In the case of isolated cells, it is the t-SNAREs microdomains rather than calcium channels that define the sites of exocytosis. (C) 2006 Elsevier Ltd. All rights reserved.
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