Eicosapentaenoic acid confers neuroprotection in the amyloid-β challenged aged hippocampus

Among the changes that occur in the hippocampus with age, is a deficit in long-term potentiation (LTP). This impairment is associated with inflammatory changes, which are typified by increased concentration of the pro-inflammatory cytokine interleukin-1 beta (IL-1 beta). Activated microglia are the most likely cell source of IL-1 beta, but data demonstrating an age-related increase in microglial activation is equivocal. Here we demonstrate that the age-related deficit in LTP is accompanied by increased expression of cell surface markers of activated microglia (major histocompatibility complex II and CD40) and increased IL-1 beta production, and that these changes may be stimulated by interferon-gamma. Treatment of aged rats with eicosapentaenoic acid (EPA) attenuates these changes and we suggest that IL-4 mediates the action of EPA. We demonstrate that aged rats exhibit an exaggerated response to intracerebroventricular injection of beta-amyloid peptide 1-40 (A beta). Thus A beta inhibited LTP in aged, but not young, rats and induced a further increase in hippocampal IL-1 beta concentration. Of particular significance is the demonstration that EPA protects the aged brain so that the increased vulnerability to A beta is ameliorated in EPA-treated rats. (c) 2006 Elsevier Inc. All rights reserved.