期刊
ANALYTICAL METHODS
卷 5, 期 21, 页码 6076-6080出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3ay41376d
关键词
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资金
- NSFC [20805011, 20905021, 21175118, 21275130, 21275131]
- Foundation of the Ministry of Education of China for Returned Scholars
Water-soluble fluorescent Ag nanoclusters (NCs) were prepared with the assistance of commercially available polyinosinic acid (PI) as a template, which can be greatly quenched by trace biological thiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH). The developed PI-Ag NCs were used for highly sensitive and selective detection of the thiols with the linear ranges of 0.1-5 mu M (R-2 = 0.9962) for Cys, 0.5-4 mu M (R-2 = 0.9967) for Hcy, and 0.05-6 mu M (R-2 = 0.9958) for GSH, respectively. This method was extended for the detection of total thiols in human plasma samples with acceptable recovery from 95.21% to 101.60% and satisfactory relative standard deviations (<5%).
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