A strip-based immunoassay for rapid determination of fenpropathrin

We report the generation of monoclonal antibodies against fenpropathrin, originating from a BALB/ c mouse immunized with a conjugate of hapten 1 [(RS)-cyano-3-phenoxybenzyl-2,2,3,3-tetramethylcyclopropane- carboxylic acid)] and keyhole limpet hemocyanin and the establishment of a monoclonal antibody-based immunochromatographic test strip. A coating antigen (test band) and a goat anti-mouse antibody (control band) were separately immobilized on nitrocellulose membrane strips as capture reagents. The anti-fenpropathrin monoclonal antibody was labeled with gold nanoparticles as a detection probe. This test strip had a wide linear range (15.6-250 mu g L-1) with a low detection limit of 62 +/- 6 mg L-1 for fenpropathrin, evaluated using a strip reader, and could be read within 10 min. This novel test strip was used to test fenpropathrin in fortified samples and found to have a high recovery rate (> 80%). In summary, our monoclonal antibody-based immunochromatographic strip offers a rapid screening tool for fenpropathrin detection in agricultural commodities.