期刊
ANALYTICAL METHODS
卷 5, 期 18, 页码 4585-4590出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3ay40931g
关键词
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资金
- National Basic Research Priorities Program [2012CB910601, 2013CB911201]
- National Natural Science Foundation of China [21075022, 21275033, 21105016]
- Fudan University
- Research Fund for the Doctoral Program of Higher Education of China [20110071110007, 20100071120053]
- Shanghai Municipal Natural Science Foundation [11ZR1403200]
- Shanghai Leading Academic Discipline Project [B109]
Crotonaldehyde has long been regarded as an important environmental pollutant from cigarette smoke to human health. To have an efficient and high-throughput screening of crotonaldehyde in saliva samples, it is essential to develop an analytical method considering both enrichment and measurement processes. In this study, we proposed a novel method based on matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for the analysis of crotonaldehyde in saliva. Magnetic graphene was synthesized via a facile hydrothermal process, and then applied as an adsorbent and a matrix for MALDI-TOF-MS. Crotonaldehyde in saliva was derived by 2,4-dinitrophenylhydrazine first followed by a magnetic solid-phase extraction process and high-throughput MALDI-TOF-MS detection. Due to its large surface area, strong interaction force and excellent inherent properties, magnetic graphene showed a satisfactory ionization and enrichment performance for the enrichment and ionization of crotonaldehyde by MALDI-TOF-MS. The crotonaldehyde derivatives could be sensitively detected from saliva samples even at a low concentrations. The results indicated that the proposed method was simple, rapid, sensitive and efficient for the analysis of crotonaldehyde in saliva and other biological samples.
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