期刊
ANALYTICAL METHODS
卷 5, 期 11, 页码 2866-2872出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3ay26599d
关键词
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资金
- National Natural Science Foundation of China [21075078]
- Natural Science Foundation of Shandong province, China [ZR2010BM005]
We have fabricated a sensitive acetylcholinesterase biosensor that is based on dual-signal amplification. A large amount of enzyme was immobilized on a glassy carbon electrode via specific binding between functionalized ZnSe quantum dots and acetylcholinesterase, and a graphene-chitosan nanocomposite was introduced that improves the response. These two factors render the biosensor highly sensitive to acetylthiocholine chloride. Organophosphate pesticides were detected with this biosensor using methyl parathion as a model enzyme inhibitor. Under optimal conditions, there is a linear relationship between the percentage of inhibition (I%) and the log of the concentration of methyl parathion in the 0.5 nM to 0.5 mu M range, with a 0.2 nM detection limit (at an S/N of 3). The biosensor displays acceptable reproducibility and relatively good storage stability. It was successfully employed to the determination of methyl parathion in spiked water and soil samples.
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