期刊
FASEB JOURNAL
卷 21, 期 8, 页码 1647-1654出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.06-6505com
关键词
endothelial progenitor cells; nanoparticles; contrast agent
资金
- NCI NIH HHS [CO-37007, U54-CA-119342] Funding Source: Medline
- NHLBI NIH HHS [HL-078631, HL-073646] Funding Source: Medline
- NIDDK NIH HHS [R01 DK053041-08, R01 DK061848, R01 DK053041, R01 DK061848-06A1] Funding Source: Medline
MRI has been employed to elucidate the migratory behavior of stem/progenitor cells noninvasively in vivo with traditional proton (H-1) imaging of iron oxide nanoparticle- labeled cells. Alternatively, we demonstrate that fluorine (F-19) MRI of cells labeled with different types of liquid perfluorocarbon ( PFC) nanoparticles produces unique and sensitive cell markers distinct from any tissue background signal. To define the utility for cell tracking, mononuclear cells harvested from human umbilical cord blood were grown under proendothelial conditions and labeled with nanoparticles composed of two distinct PFC cores ( perfluorooctylbromide and perfluoro-15-crown-5 ether). The sensitivity for detecting and imaging labeled cells was defined on 11.7T ( research) and 1.5T ( clinical) scanners. Stem/progenitor cells (CD34(+) CD133(+) CD31(+)) readily internalized PFC nanoparticles without aid of adjunctive labeling techniques, and cells remained functional in vivo. PFC-labeled cells exhibited distinct F-19 signals and were readily detected after both local and intravenous injection. PFC nanoparticles provide an unequivocal and unique signature for stem/progenitor cells, enable spatial cell localization with F-19 MRI, and permit quantification and detection of multiple fluorine signatures via F-19 MR spectroscopy. This method should facilitate longitudinal investigation of cellular events in vivo for multiple cell types simultaneously.
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