期刊
ANALYTICAL METHODS
卷 3, 期 2, 页码 299-305出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0ay00423e
关键词
-
资金
- Promotion and Mutual Aid Corporation for Private Schools of Japan
- Science Research Promotion Fund
- [19590049]
Tea products and crude drugs were analyzed to determine levels of contamination with 15 polycyclic aromatic hydrocarbons (PAHs) using a new online automated method, consisting of in-tube solid-phase microextraction (SPME) coupled with high-performance liquid chromatography-fluorescence detection (HPLC-FLD). PAHs were separated within 15 min by HPLC using a Zorbax Eclipse PAH column with a water/acetonitrile gradient elution program as the mobile phase. The optimum in-tube SPME conditions were 20 draw/eject cycles of 40 mu L of sample using a CP-Sil 19CB capillary column as an extraction device. Low- and high-molecular weight PAHs were extracted effectively onto the capillary coating from 5% and 30% methanol solutions, respectively. The extracted PAHs were readily desorbed from the capillary by passage of the mobile phase. Good linearity of the calibration curve (r > 0.9972) was obtained in the concentration range of 0.05-2.0 ng mL(-1), and the detection limits (S/N = 3) of PAHs were 0.32-4.63 pg mL(-1). Using the in-tube SPME/HPLC-FLD method, PAHs were detected at ng g(-1) levels in the samples without interference peaks. Our results suggest that PAHs contamination in herbal products is widespread, and the proposed method may become a useful tool for monitoring PAH contamination and quality control in herbal products.
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