期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 16, 期 12, 页码 28647-28656出版社
MDPI AG
DOI: 10.3390/ijms161226118
关键词
grouper iridovirus of Taiwan (TGIV); major capsid protein (MCP); recombinant vaccine
资金
- Fisheries Agency of the Council of Agriculture, Executive Yuan
- Center of Excellence for the Oceans of National Taiwan Ocean University
- Ministry of Science and Technology in Taiwan [MOST 104-2321-B-019-004-MY3]
Fish iridoviruses cause systemic diseases with high mortality in various species of wild and farm-raised fish, resulting in severe economic losses. In 1998, we isolated a new epizootic iridovirus in cultured grouper (Epinephelus sp.) in Taiwan, thus named as grouper iridovirus of Taiwan (TGIV). We report here the cloning of TGIV major capsid protein (MCP). Phylogenetic analysis of the iridoviral MCPs confirmed the classification of TGIV into the Megalocytivirus genus. Recombinant TGIV MCP and GIV MCP were then generated to produce polyclonal antibodies. Western blot analysis revealed that the two antisera were species-specific, indicating no common epitope shared by the MCPs of the two viruses. We further assayed the potency of a subunit vaccine containing recombinant TGIV MCP. The vaccine effectively protected grouper from TGIV infection. The result demonstrated that MCP is a suitable antigen for anti-TGIV vaccines.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据