期刊
ANALYTICAL METHODS
卷 2, 期 9, 页码 1320-1332出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0ay00288g
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资金
- NIH [1R03 HL095419-01A1]
- FAMRI [062415]
- US Army [W81XWH-06-2-0044]
- National Heart, Lung, and Blood Institute (NHLBI)
We report the validation of a reversed-phase gradient HPLC method allowing simultaneous quantification of retinol, retinyl esters, tocopherols and selected carotenoids in lung, liver and plasma of mouse, rat and guinea pig (gp) using a diode array detector. A significant species difference was observed regarding the distribution of retinol and retinyl esters. The levels of total retinol in lung, liver and plasma were in the following order: mouse >> rat > gp; rat > mouse > gp; and gp >> rat > mouse, respectively. Furthermore, comparison studies revealed similarities between the vitamin A profiles of human and gp lung samples.
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