期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 103, 期 6, 页码 535-541出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1263/jbb.103.535
关键词
solid-state cultivation; crab shellfish waste; chitinase; chitin-saccharification; Aspergillus sp S1-13
in a suspension of solid-state culture of Aspergillus sp. Sl-13 containing a lactic acid-treated crab shell as the substrate, the saccharification of chitin in the shell proceeded to form N-acetyl-glucosamine (GIcNAc): the culture was the source of chitin and chitinases. The analysis of chitinases in the water-extract of the solid-state culture indicated occurrence of an exochitinase (Exo, MW 73 kDa) and two endochitinases. The amounts of the endochitinases suggested that one of them (Endo-1, MW 45 kDa) might be the main species in the chitin-saccharification. The amount of GlcNAc released from the LA-treated crab shell by the combined action of isolated Exo and Endo-1 was very small, predicting participation in the saccharification of other enzyme species, which might be hardly extracted with water from the solid-state culture. The re-extraction of the solid-state culture using 2 M KC1, which was extracted with water beforehand, demonstrated another endochitinase (Endo-2, MW 51 kDa). Endo-2 isolated from the salt-extract can adsorb to chitin, and can hydrolyze the chitin in the adsorbed state. The roles of these chitinases in the chitin-saccharification based on their properties and combined action were discussed.
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