期刊
MOLECULAR AND CELLULAR PROBES
卷 21, 期 3, 页码 202-207出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2006.12.001
关键词
catechol-O-methyltransferase; human polymorphism; SNP; amplification refractory mutation system (ARMS)
The G -> A transition at nucleotide 21881 of the human catechol-O-niethyltramsferase (COM7 gene represents a functional genetic polymorphism (Va1158Met), rendering an enzyme with reduced activity that has been associated with psychiatric disorders and estrogen related cancers. A new method for the detection of this polymorphism is described, based on the tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), with a single PCR to discriminate both alleles. Two primers amplify a common amplicon independently of the allele considered. At the same time, two primers are used, differing in the 3' base. In the Val/Val or Met/Met conditions, amplification occurs both in the general amplicon and in the specific allele; in the Val/Met condition three different amplicons are produced. Direct DNA sequencing of a COMT region containing the G/A polymorphism demonstrates the validity of this tetra-primer ARMS-PCR method. Reevaluation by PCR-RFLP revealed 100% accordance for genotype adscription. Subjects carrying the COMTHH genotype in a Spanish population comprised 28%, and the COMLL homozygotes amounted to 21%. The described method provides a fast and reliable approach for determining COMT polymorphism that can be useful in large clinical studies using minimal quantity of DNA, avoiding the timely and costly use of restriction enzymes. (c) 2007 Elsevier Ltd. All rights reserved.
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