期刊
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卷 45, 期 16, 页码 2300-2309出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2012.686132
关键词
Chitosan; Epigallocatechin gallate; Molecularly imprinted polymer; Separation and purification
资金
- Fujian Provincial Natural Science Foundation [2010J01025]
- Fujian Education Department [JB09240]
A molecularly imprinted polymer (MIP), which was suitable for recognizing epigallocatechin gallate (EGCG), was prepared by using EGCG as template molecule and biocompatible chitosan as a functional matrix in aqueous medium. Molecular recognition ability of the EGCG-imprinted polymer (EIP) was evaluated by high performance liquid chromatography (HPLC). The results show that the EIP has a high imprinting factor (1.32) for EGCG and was used to purify EGCG from crude tea polyphenol efficiently. The percentage of EGCG can be improved from 78.6% in crude tea polyphenol (TP) to 90.1% in product and the adsorption quantity per unit can reach 4.02 mg.g(-1). EIP shows potential excellent prospect in the application of separating and purifying EGCG from TP.
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