期刊
ANALYTICAL LETTERS
卷 44, 期 16, 页码 2633-2643出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2011.553009
关键词
Molecularly imprinted polymers; Mycotoxin production; Precipitation polymerization; Zearalenone
资金
- Belgian Federal Science Policy Office (BELSPO) [BL/02/c46]
- National 863'' High-Tech Project of China [2007AA10Z436, 2008AA100804]
In this work, molecularly imprinted polymers (MIPs) using the mycotoxin zearalenone (ZEA) as template were first synthesized via precipitation polymerization and then applied as specific adsorbents in molecularly imprinted solid-phase extraction (MISPE) cartridges. The MISPE procedure was optimized with 3 mL of acetonitrile for preconditioning, 1 mL of acetonitrile:H(2)O (60:40) for loading, 1 mL of acetonitrile:H(2)O (30:70), and 3 mL of methanol:acetic acid (95:5) for elution. The obtained MIPs showed high selectivity of 96.9% towards ZEA, and low cross-reactivity (1-20%) to other Fusarium mycotoxins including deoxynivalenol, nivalenol, HT-2 toxin and T-2 toxin. The cross-reactivity to fumonisin B(1) amounted to 61%. The MISPE was applied for enrichment of ZEA, which was produced by Fusarium graminearum strains. An enrichment factor above 50 was reached. Recoveries of 1 mu g/mL were between 90.8% and 99.6%. A small amount of ZEA was produced by 9 F. graminearum strains with a maximum of 13 mu g, then purified by the developed MISPE and analyzed by LC-MS/MS.
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