PREPARATION OF MOLECULARLY IMPRINTED POLYMER FOR EXTRACTING FLAVONES FROM CHAMAECYPARIS OBTUSA

A quercitrin, molecularly imprinted polymer with specific recognition ability for flavone was prepared using quercitrin as template, acrylamide as functional monomer, and ethylene glycol dimethacrylate as cross-linker in the porogen of tetrahydrofuran. The preparation procedure was optimized and the retention behavior and selectivity of this molecularly imprinted SPE were evaluated by HPLC. Crude extract of flavones from C. obtusa was purified by using this polymer as the separation medium. The mean recoveries of quercitrin, myricetin, and amentoflavone were 78.0, 88.0, and 72.0%, and the detection limits for quercitrin, myricetin, and amentoflavone were 0.05, 0.02, and 0.7 mu g mL(-1), respectively.