期刊
ANALYTICAL LETTERS
卷 44, 期 6, 页码 1085-1099出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2010.511736
关键词
Antibody array; Chemiluminescence; E. coli O157:H7; Immunoassay; Salmonella spp. milk
资金
- National Center for Genetic Engineering and Biotechnology (BIOTEC, Thailand)
- NanoASIA, LLc. (Thailand)
A foodborne antibody array (FAbA) was developed as a rapid, inexpensive, and accurate detection to simultaneously detect Escherichia coli O157:H7 and Salmonella spp. using a custom-made chemiluminescent detector. We have optimized fabrication processes, selected the antibody concentrations, calibrated the signals to the pathogen amount, and minimized assay time. In comparison with 4 hours for ELISA, FAbA takes only 75 minutes to achieve relatively the same sensitivities (8x104 and 5x107CFU/ml for E. coli and Salmonella, respectively). The FAbA was able to detect the pathogens in milk at the same detection limits as in the buffer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据