DETERMINATION OF VITAMINS A AND E EXPLOITING CLOUD POINT EXTRACTION AND MICELLAR LIQUID CHROMATOGRAPHY

Cloud point extraction and micellar chromatographic methods were developed for determination of vitamins A and E. The stationary phase was C18 and the mobile phase was 3.00% (w/v) SDS, 15.0% (v/v) butyl alcohol and 0.02 mol L-1 phosphate buffer solution at pH 7.0. The retention times for vitamins A and E were 9.6 and 13.0, respectively. The extraction solution was 100 mmol L-1 Triton X-100, 650 mg NaCl and 1.0% ascorbic acid at 70 degrees C for 30 min. The method is precise (r.s.d.< 7%), the linear range was from 5.0 up to 360.0 mg L-1 for both vitamins. Recovery test showed recuperation between 90.2 and 99.2%, and LOD and LOQ of 0.234 and 0.108mg L-1, 0.780 and 0.360 mg L-1 to vitamins A and E were found.