期刊
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
卷 13, 期 2, 页码 137-140出版社
SPRINGER
DOI: 10.1007/s11655-007-0137-2
关键词
CD18 adhesion molecule; acute lung injury; tanshinone IIA; malondialdehyde; coagulation abnormality
Objective: To explore the protective effect of tanshinone II A on lipopolysaccharide (LPS)-induced lung injury in rats, and possible mechanism. Methods: LPS(O-111:B4) was used to produce a rat model of acute lung injury. Sprague-Dawley rats were randomly divided into 3 groups (8 in each group): the control group, the model group (ALI group), and the tanshinone II A treatment group. Expression of adhesion molecule CD18 on the surface of polymorphonuclear neutrophil (PMN-CD18) in venous white blood cells (WBC), and changes in coagulation-anticoagulant indexes were measured 6 h after injection of LPS or normal saline. Changes in malondialdehyde (MDA) content, wet and dry weight (W/D) ratio and morphometry of pulmonary tissue as well as PMN sequestration in the lung were also measured. Results: (1) When compared with the control group, expression of PMN-CD18 and MDA content were enhanced in the ALI group with a hypercoagulable state (all P < 0.01) and an increased W/D ratio (P < 0.05). Histopathological morphometry in the lung tissue showed higher PMN sequestration, wider alveolar septa; and lower alveolar volume density (V-V) and alveolar surface density (S-V), showing significant difference (P < 0.01). (2) When compared with the ALI group, the expression of PMN-CD18, MDA content, and W/D ratio were all lower in Tanshinone II A treatment group (P < 0.05) with ameliorated coagulation abnormality (P < 0.01). Histopathological morphometry in the lung tissue showed a decrease in the PMN sequestration and the width of alveolar septa (both P < 0.01), and an increase in the V-V and S-V (P < 0.05, P < 0.01). Conclusion: Tan II A plays a protective role in LPS-incluced lung injury in rats through improving hypercoagulating state, decreasing PMN-CD18 expression and alleviating migration, reducing lipid peroxidation and alleviating pathological changes.
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