IDENTIFICATION AND SIMULTANEOUS DETERMINATION OF MANGIFERIN, NEOMANGIFERIN, TIMOSAPONIN A-III, AND TIMOSAPONIN C IN RHIZOMA ANEMARRHENAE BY RAPID RESOLUTION LIQUID CHROMATOGRAPHY COUPLED WITH TRIPLE QUADRUPOLE MASS SPECTROMETRY

A new and rapid method was developed for simultaneous determination of mangiferin, neomangiferin, timosaponin A-III, and C in Rhizoma Anemarrhenae using rapid resolution liquid chromatography coupled with triple quadrupole mass spectrometry. The analysis was performed on an Eclipse Plus C-18 column (I. D. 4.6 x 100 mm, 3.5 mu m). Electrospray ionization-tandem interface in the negative mode was employed prior to mass spectrometric detection. Quantitation was based on multiple reaction monitoring (MRM) for determination. Limits of detection (LODs) ranged from 0.7 to 3 pg. The average recoveries ranged from 98.16 to 100.7% with RSDs <= 2.03%. The established method was validated, sensitive, and reliable.