DETECTION OF BIOGENIC AMINES IN HUMAN SALIVA USING A SCREEN-PRINTED BIOSENSOR

An electrochemical biosensor to detect biogenic amines in saliva was developed. This biosensor is based on a Prussian Blue screen-printed electrode, which senses the H(2)O(2) produced by the reaction catalyzed by the diamine oxidase enzyme. The probe was connected with a portable instrument and chronoamperometric drop measurements were performed. After evaluation of the biosensor's ability to interact with different amines, putrescine was chosen as the reference amine. A detection limit of 1 x 10(-5) M and a working range between 2 x 10(-5) and 3 x 10(-4) M were obtained. Once optimized, the saliva treatment, a negligible matrix effect, and an average recovery of 105% were obtained.