期刊
ANALYTICAL LETTERS
卷 43, 期 2, 页码 280-288出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710903325815
关键词
2-(2-thienyl)benzothiazoline; Fluorescent probe; Spectrofluorometric method; Superoxide anion radical; Superoxide dismutase
A new fluorescent probe, 2-(2-thienyl)benzothiazoline was synthesized. Its structure was characterized with elemental analysis, IR and H-1 NMR. The fluorescent probe can be oxidized by O-2(center dot-) to form a less fluorescent product. Based on this reaction, a spectrofluorometric method was developed to determine the content of superoxide anion radicals and superoxide dismutase activity. There was a good linear relationship with a correlation coefficient 0.9996 between the scavenging percentage of O-2(center dot-) and SOD activity. The method was simple, selective, and sensitive. It was successfully applied to the determination of SOD activity in garlic and onion samples.
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