期刊
FEBS JOURNAL
卷 274, 期 11, 页码 2749-2765出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1742-4658.2007.05809.x
关键词
cell signaling; CREB; FOXO; gene expression; mitochondria
资金
- NHLBI NIH HHS [R01 HL068698-05, HL 68698] Funding Source: Medline
Lithium is a therapeutic agent commonly used to treat bipolar disorder and its beneficial effects are thought to be due to a combination of activation of the Wnt/beta-catenin pathway via inhibition of glycogen synthase kinase-3 beta and depletion of the inositol pool via inhibition of the inositol monophosphatase-1. We demonstrated that lithium in primary endothelial cells induced an increase in mitochondrial mass leading to an increase in ATP production without any significant change in mitochondrial efficiency. This increase in mitochondrial mass was associated with an increase in the mRNA levels of mitochondrial biogenesis transcription factors: nuclear respiratory factor-1 and -2 beta, as well as mitochondrial transcription factors A and B2, which lead to the coordinated upregulation of oxidative phosphorylation components encoded by either the nuclear or mitochondrial genome. These effects of lithium on mitochondrial biogenesis were independent of the inhibition of glycogen synthase kinase-3 beta and independent of inositol depletion. Also, expression of the coactivator PGC-1 alpha was increased, whereas expression of the coactivator PRC was not affected. Lithium treatment rapidly induced a decrease in activating Akt-Ser473 phosphorylation and inhibitory Forkhead box class O (FOXO1)-Thr24 phosphorylation, as well as an increase in activating c-AMP responsive element binding (CREB)-Ser133 phosphorylation, two mechanisms known to control PGC-1 alpha expression. Together, our results show that lithium induces mitochondrial biogenesis via CREB/PGC-1 alpha and FOXO1/PGC-1 alpha cascades, which highlight the pleiotropic effects of lithium and reveal also novel beneficial effects via preservation of mitochondrial functions.
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