FT-IR Spectrophotometric Analysis of Ascorbic Acid and Biotin and their Pharmaceutical Formulations

Fourier transform-infrared (FT-IR) spectrometry was used for the rapid, direct measurement of ascorbic acid (vitamin C) and biotin (vitamin H) in different pharmaceutical products. Conventional KBr spectra were compared for the best determination of active substances in drug preparations. The Beer-Lambert law and chemometric approaches were applied in data processing.