期刊
ANALYTICAL LETTERS
卷 42, 期 17, 页码 2893-2904出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710903201909
关键词
Abscisic acid (ABA); gold nanoparticles; immunoreaction; in situ chemical reduction
资金
- National Natural Science Foundation of China [90817101, 30670190, 30600049]
- National Commonwealth Research Project of China for Quality Supervision, Inspection and Quarantine [2007GYB146]
An amperometric immunosensor for phytohormone abscisic acid was developed based on in situ chemical reductive growth of gold nanoparticles on glassy carbon electrode. First, an approximate 10nm gold layer was sputtered uniformly onto the electrode surface, and then gold nanoparticles were grown directly on the gold layer for antibody adsorption by immersing the electrode into the H2AuCl4 solution. Determination was based on an enzyme-linked competitive immunoreaction between free and enzyme-labeled abscisic acid to bind on immobilized antibody on electrode. The linear response was from 10ng/ml to 10 mu g/ml with a detection limit of 5ng/ml.
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