期刊
ANALYTICAL LETTERS
卷 41, 期 12, 页码 2224-2236出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710802238150
关键词
chitosan; direct electron transfer; gold nanoparticles; horseradish peroxidase; ITO electrode
资金
- National Natural Science Foundation of China [50671098, 20473091, 20573112]
Gold nanoparticles/chitosan modified indium tin oxide (ITO) coated glass electrode was prepared by a simple layer-by-layer self-assembly technique. Horseradish peroxidase (HRP) was successfully immobilized on the modified electrode by electrostatic adsorption. Direct electrochemistry and electrocatalysis of HRP were investigated. The composite film showed an obvious promotion for the direct electron transfer between HRP and the underlying electrode. The immobilized HRP exhibited an excellent electrocatalytic activity toward the reduction of H2O2. The catalysis currents increased linearly to the H2O2 concentration in a wide range of 2.0 x 10(-5) to 6.5 x 10(-3) M with a low detection limit of 3.5 x 10(-6) M. The calculated apparent Michaelis-Menten constant (K-m(app)) was 4.43mM.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据