期刊
ANALYTICAL LETTERS
卷 41, 期 14, 页码 2554-2563出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710802363131
关键词
Human serum; multiwalled carbon nanotubes; protein enrichment; SELDI-TOF-MS
资金
- Capital Normal University [07224010039]
The present paper describes a biomarker capturing strategy based on the enrichment efficiency of multiwalled carbon nanotubes (MWCNTs) for peptides and proteins from human serum. The method is shown to enrich proteins/peptides, and the captured molecules were profiled using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Mass spectra of the MWCNTs-treated human serum samples showed a significant enrichment of proteins by MWCNTs. Preliminary results indicated a good level of reproducibility in serum profiles with coefficients of variation (CVs) on signal intensity ranging from 8.9% to 25.2%. The developed method holds promise for improving the discovery of low-abundance serum biomarkers.
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