Activation of double-stranded DNA by one pcPNA strand for its site-selective scission with CeIV/EDTA

In the presence of only one strand of pseudo-complementary PNA (pcPNA), double-stranded DNA was selectively hydrolyzed by Ce-IV/EDTA (EDTA = ethylenediamine-N,N,N ',N '-tetraacetate) at target site. Compared with previously reported artificial restriction DNA cutters using two pcPNA strands, the present cutters are simple and promising for various applications.