期刊
NEUROSCIENCE
卷 146, 期 4, 页码 1618-1628出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2007.03.002
关键词
knockout mice; brain development; nicotinic receptors
The nicotinic receptor subtypes are important for several physiological functions in brain and may therefore play a critical role in brain development. The alpha 7 nicotinic receptors which have high Ca2+ permeability are important for cognitive, neuroprotective and trophic functions. In this study, the brain development and the expression of alpha 4, alpha 3, alpha 7, alpha 5 and beta 2 nicotinic receptors were investigated in the brains of alpha 7 deficient (alpha 7 -/-), alpha 7 heterozygous null (alpha 7 +/-) and alpha 7 wild-type (alpha 7 +/+) mice from postnatal days (P) 7-84. The specific binding of [H-3] cytisine and [H-3] epibatidine, as well as the expressions of alpha 4 and alpha 3 nicotinic receptor subunits at mRNA and protein levels, were significantly increased in the cortex and hippocampus of alpha 7 -/- and alpha 7 +/- mice compared with alpha 7 +/+ mice. Furthermore, the alpha 4 and alpha 3 nicotinic acetylcholine receptor (nAChR) subunits appeared to co-assemble with the alpha 5 nAChR subunit in these above brain regions of these mice. No significant change in synaptophysin level was observed. These data suggest that increased levels of alpha 4, alpha 3-containing nAChRs, co-assembled with the alpha 5 nAChR subunit, may contribute to the normal brain development of alpha 7 -/- and alpha 7 +/- mice. (C) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据