期刊
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
卷 70, 期 4, 页码 565-572出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbbm.2007.01.001
关键词
BN-PAGE; ammonium sulfate precipitation; peptide mass fingerprint; protein identification; Streptomyces coelicolor
Ammonium sulfate precipitation was tested as a sample preparation step for BN-PAGE analyses of S. coelicolor cytoplasmic protein complexes. A procedure of sample preparation compatible with two-dimensional BN/SDS-PAGE was established and used to visualize protein complexes. To validate the sample preparation procedure, representative protein complexes were identified. Several previously characterized protein complexes were rediscovered and their reported oligomeric states reconfirmed. In addition, we identified new but plausible interactions that have never been reported before. Our work provides useful reference for the wide application of BN-PAGE in protein interaction study. (C) 2007 Elsevier B.V. All rights reserved.
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