期刊
ANALYTICAL CHEMISTRY
卷 90, 期 17, 页码 10171-10178出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b00254
关键词
-
资金
- Global Research Laboratory (GRL) Program [NRF-2013K1A1A2A02050616]
- Midcareer Researcher Program through a National Research Foundation of Korea (NRF) - Ministry of Science and ICT [NRF-2017R1A2B3010816]
- World Institute of Kimchi - Ministry of Science and ICT, Republic of Korea [KE1701-5]
Bacterial infections are common causes of morbidity and mortality worldwide; therefore, environmental contamination by bacterial pathogens represents a global public health concern. Consequently, a selective, rapid, sensitive, and in-field detection platform for detecting significant bacterial contamination is required to ensure hygiene and protect public health. Here, we developed a fast and simple platform for the selective and sensitive detection of bacteria by measuring adenosine triphosphate (ATP) bioluminescence following targeted photothermal lysis mediated by antibody conjugated gold nanorods. This method employed both targeted photo thermal lysis of bacteria by near-infrared (NIR) irradiation and highly selective detection of the lysed bacteria via ATP bioluminescence within 36 min (incubation, 30 min; NIR irradiation, 6 min). The use of the proposed method allowed limits of detection in pure solution of 12.7, 70.7, and 5.9 CFU for Escherichia coli O157:H7, Salmonella typhimurium, and Listeria monocytogenes, respectively. Additionally, bacteria were successfully detected on artificially inoculated plastic cutting boards. Furthermore, this method was highly specific, without cross-reaction among pathogenic bacteria. We believe that the proposed method has significant potential as an on-site diagnostic tool for applications associated with public health and environmental pollution monitoring.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据