期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 124, 期 1, 页码 172-178出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2006.12.019
关键词
nanoparticles; xi-potential; fluorescence
资金
- NIEHS NIH HHS [P42 ES004699-180016, P42 ES004699] Funding Source: Medline
Increasingly growing application of nanoparticles in biotechnology requires fast and accessible tools for their manipulation and for characterization of their colloidal properties. In this work we determine the zeta-potentials for polystyrene nanoparticles using micro-electrical field flow fractionation (mu-EFFF) which is an efficient method for sorting particles by size. The data obtained by mu-EFFF were compared to -potentials determined by standard capillary electrophoresis. For proof of concept, we used polystyrene nanoparticles of two different sizes, impregnated with two different fluorescent dyes. Fluorescent emission spectra were used to evaluate the particle separation in both systems. Using the theory of electrophoresis, we estimated the zeta-potentials as a function of size, dielectric permittivity, viscosity and electrophoretic mobility. The results obtained by the mu-EFFF technique were confirmed by the conventional capillary electrophoresis measurements. These results demonstrate the applicability of the mu-EFFF method not only for particle size separation but also as a simple and inexpensive tool for measurements of nanoparticle zeta-potentials. (c) 2006 Elsevier B.V. All rights reserved.
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