期刊
CARBOHYDRATE RESEARCH
卷 342, 期 8, 页码 1030-1033出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2007.02.008
关键词
agarose; enzymatic hydrolysis; neoagaro-oligosaccharides; recombinant beta-agarase; AgaA; AgaB
In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner, an enzymatic degradation method was developed, which includes degradation with either recombinant beta-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography. Agarose was degraded with AgaA at the optimized conditions, yielding 47% and 45% of neoagarotetraose and neoagarohexaose, respectively. These neoagaro-oligosaccharides were conveniently separated by consecutive column chromatography on Bio-Gel P2 or P6 and were identified by FACE. The structure of these neoagaro-oligosaccharides was confirmed by MALDI-TOF MS and C-13 NMR spectroscopy. (c) 2007 Elsevier Ltd. All rights reserved.
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