期刊
ANALYTICAL CHEMISTRY
卷 79, 期 12, 页码 4666-4673出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac0618730
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资金
- NCRR NIH HHS [P41 RR11823-10, P41 RR011823-11, P41 RR011823] Funding Source: Medline
- NIMH NIH HHS [R01 MH067880, 5R01 MH067880-02, R01 MH067880-06] Funding Source: Medline
An automated online multidimensional liquid chromatography system coupled to ESI-based tandem mass spectrometry was used to assess the effectiveness of TiO2 in the enrichment of phosphopeptides from tryptic digests of protein mixtures. By monitoring the enrichment of phosphopeptides, an optimized set of loading, wash, and elution conditions were realized for TiO2. A comparison of TiO2 with other resins used for phosphopeptide enrichment, Fe(III)-IMAC and ZrO2, was also carried out using tryptic digests of both simple and moderately complex protein mixtures; where TiO2 was shown to be superior in performance.
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