期刊
ANALYTICAL CHEMISTRY
卷 86, 期 13, 页码 6572-6579出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac501088q
关键词
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资金
- National Natural Science Foundation of China [21190040, 21221003, 21175035]
- National Basic Research Program [2011CB911002]
- International Science & Technology Cooperation Program of China [2010DFB30300]
- Fundamental Research Funds for the Central Universities
An aptamer screening method using a positive and negative selection units integrated microfluidic chip was introduced. Here, myoglobin (Myo), one of the early markers to increase after acute myocardial infarction, was used as the model. After 7-round selection, the aptamers, which exhibited dissociation constants (K-d) in the nanomolar range (from 4.93 to 6.38 nM), were successfully obtained using a positive and negative selection units integrated microfluidic chip. The aptamer with the highest affinity (K-d = 4.93 nM) was then used for the fabrication of a label-free supersandwich electrochemical biosensor for Myo detection based on target-induced aptamer displacement. The detection limit of this aptamer-based electrochemical biosensor was 10 pM, which was significantly lower than that of those previous antibody-based biosensors for Myo detection. This work may not only develop a strategy for screening aptamer but also offer promising alternatives to the traditional analytical and immunological methods for Myo detection.
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