期刊
ANALYTICAL CHEMISTRY
卷 86, 期 3, 页码 1632-1639出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac403384n
关键词
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资金
- National Institutes of Health [R01 DK091538, R01 ES022181, L30 AG0 038036]
- Children's Discovery Institute of the St. Louis Children's Hospital Foundation
Studies of isotopically labeled compounds have been fundamental to understanding metabolic pathways and fluxes. They have traditionally, however, been used in conjunction with targeted analyses that identify and quantify a limited number of labeled downstream metabolites. Here we describe an alternative workflow that leverages recent advances in untargeted metabolomic technologies to track the fates of isotopically labeled metabolites in a global, unbiased manner. This untargeted approach can be applied to discover novel biochemical pathways and characterize changes in the fates of labeled metabolites as a function of altered biological conditions such as disease. To facilitate the data analysis, we introduce (XCMS)-C-13, an extension of the widely used mass spectrometry-based metabolomic software package XCMS. (XCMS)-C-13 uses the XCMS platform to detect metabolite peaks and perform retention-time alignment in liquid chromatography/mass spectrometry (LC/MS) data. With the use of the XCMS output, the program then identifies isotopologue groups that correspond to isotopically labeled compounds. The retrieval of these groups is done without any a priori knowledge besides the following input parameters: (i) the mass difference between the unlabeled and labeled isotopes, (ii) the mass accuracy of the instrument used in the analysis, and (iii) the estimated retention-time reproducibility of the chromatographic method. Despite its name, (XCMS)-C-13 can be used to track any isotopic label. Additionally, it detects differential labeling patterns in biological samples collected from parallel control and experimental conditions. We validated the ability of (XCMS)-C-13 to accurately retrieve labeled metabolites from complex biological matrices both with targeted LC/MS/MS analysis of a subset of the hits identified by the program and with labeled standards spiked into cell extracts. We demonstrate the full functionality of (XCMS)-C-13 with an analysis of cultured rat astrocytes treated with uniformly labeled (U-)C-13-glucose during lipopolysaccharide (LPS) challenge. Our results show that out of 223 isotopologue groups enriched from U-C-13-glucose, 95 have statistically significant differential labeling patterns in astrocytes challenged with LPS compared to unchallenged control cells. Only two of these groups overlap with the 32 differentially regulated peaks identified by XCMS, indicating that (XCMS)-C-13 uncovers different and complementary information from untargeted metabolomic studies. Like XCMS, (XCMS)-C-13 is implemented in R. It is available from our laboratory website at http://pattilab.wustl.edu/x13cms.php.
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