期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1153, 期 1-2, 页码 259-276出版社
ELSEVIER
DOI: 10.1016/j.chroma.2006.11.054
关键词
biomarkers; sample preparation; mass spectrometry; proteomics; serum/plasma
资金
- NCI NIH HHS [2P30CA016087-239025] Funding Source: Medline
In this article, we present an overview of the different strategies for sample preparation for identification by mass spectrometry (MS) of biomarkers from serum and/or plasma. We consider the effects of the variables involved in sample collection, handling and storage, and describe different approaches for removal of high abundance proteins and serum/plasma fractionation. We review the advantages and disadvantages of such techniques as centrifugal ultrafiltration, different formats for solid phase extraction, organic solvent extraction, gel and capillary electrophoresis, and liquid chromatography. We also discuss a variety of current proteomic methods and their main applications for biomarker-related studies. (c) 2006 Elsevier B.V. All rights reserved.
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