期刊
ANALYTICAL CHEMISTRY
卷 86, 期 15, 页码 7843-7848出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac502276w
关键词
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资金
- National Basic Research Program (973 Program) [2012CB932600]
- Chinese Academy of Sciences
- Shanghai Pujiang Project [13PJ1410700]
- National Natural Science Foundation of China [21175155]
- Hunan Provincial Innovation Foundation for Postgraduate
Sensitive detection of cancer cells plays a critically important role in the early detection of cancer and cancer metastasis. However, because circulating tumor cells are extremely rare in peripheral blood, the detection of cancer cells with high analytical sensitivity and specificity remains challenging. Here, we have demonstrated a simple, sensitive and specific detection of cancer cells with the detection sensitivity of four cancer cells, which is lower than the cutoff value with respect to correlation with survival outcomes as well as predictive of metastatic disease in clinical diagnostics. We re-engineered the hybridization chain reaction (HCR) to multibranched HCR (mHCR) that can produce long products with multiple biotins for signal amplification and multiple branched arms for multivalent binding. The capturing gold surface is modified with DNA tetrahedral probes, which provide superior hybridization conditions for the multivalent binding. The synergetic effect of mHCR amplification and multivalent binding lead to the high sensitivity of our detection platform.
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