期刊
ANALYTICAL CHEMISTRY
卷 86, 期 14, 页码 6976-6982出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac500955r
关键词
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资金
- National Natural Science Foundation of China [21175039, 21190044, 21221003, 21322509, 21305035, 21305038]
- Hunan Province Science and Technology Project of China [2013FJ4042]
- Hunan Provincial Graduate Research and Innovation Projects [CX2013B139]
DNA-templated copper nanoparticles (CuNPs) have emerged as promising fluorescent probes for biochemical assays, but the reported monomeric CuNPs remain problematic because of weak fluorescence and poor stability. To solve this problem, a novel concatemeric dsDNA-templated CuNPs (dsDNA-CuNPs) strategy was proposed by introducing the rolling circle replication (RCR) technique into CuNPs synthesis. In this strategy, a short oligonucleotide primer could trigger RCR and be further converted to a long concatemeric dsDNA scaffold through hybridization. After the addition of copper ions and ascorbate, concatemeric dsDNA-CuNPs could effectively form and emit intense fluorescence in the range of 500-650 nm under a 340 nrn excitation. In comparison with monomeric dsDNA-CuNPs, the sensitivity of concatemeric dsDNA-CuNPs was greatly improved with similar to 10 000 folds amplification. And their fluorescence signal was detected to reserve similar to 60% at 2.5 h after formation, revealing similar to 2 times enhanced stability. On the basis of these advantages, microRNA let-7d was selected as the model target to testify this strategy as a versatile assay platform. By directly using let-7d as the primer in RCR, the simple, low-cost, and selective microRNA detection was successfully achieved with a good linearity between 10 and 400 pM and a detection limit of 10 pM. The concatemeric dsDNA-CuNPs strategy might be widely adapted to various analytes that can directly or indirectly induce RCR.
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