期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 16, 期 2, 页码 3035-3057出版社
MDPI
DOI: 10.3390/ijms16023035
关键词
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资金
- National Natural Science Foundation of China [30470189, 81071821, 81250110086, 812111392]
- Key Hunan Provincial Natural Science Foundation of China [13JJ2016, 07JJ5096]
- Science and Technology Program of Hunan Province of China [2014SK4050]
- Changsha National High Tech Zone Innovation Leading Talent Program [20143701]
- Fundamental Research Funds for the Central Universities of Central South University [721500011, 2013zzts067]
The Panax ginseng C.A. Meyer belonging to the Araliaceae has long been used as an herbal medicine. Although public databases are presently available for this family, no methyl jasmonate (MeJA) elicited transcriptomic information was previously reported on this species, with the exception of a few expressed sequence tags (ESTs) using the traditional Sanger method. Here, approximately 53 million clean reads of adventitious root transcriptome were separately filtered via Illumina HiSeq (TM) 2000 from two samples treated with MeJA (Pg-MeJA) and equal volumes of solvent, ethanol (Pg-Con). Jointly, a total of 71,095 all-unigenes from both samples were assembled and annotated, and based on sequence similarity search with known proteins, a total of 56,668 unigenes was obtained. Out of these annotated unigenes, 54,920 were assigned to the NCBI non-redundant protein (Nr) database, 35,448 to the Swiss-prot database, 43,051 to gene ontology (GO), and 19,986 to clusters of orthologous groups (COG). Searching in the Kyoto encyclopedia of genes and genomes (KEGG) pathway database indicated that 32,200 unigenes were mapped to 128 KEGG pathways. Moreover, we obtained several genes showing a wide range of expression levels. We also identified a total of 749 ginsenoside biosynthetic enzyme genes and 12 promising pleiotropic drug resistance (PDR) genes related to ginsenoside transport.
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