期刊
ANALYTICAL CHEMISTRY
卷 86, 期 6, 页码 2955-2962出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac403223f
关键词
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资金
- Soliance society
- CNRS
- Ministry of Higher Education and Research
- Champagne-Ardenne DRRT
- EU
Because of their highly complex metabolite profile, the chemical characterization of bioactive natural extracts usually requires time-consuming multistep purification procedures to achieve the structural elucidation of pure individual metabolites. The aim of the present work was to develop a dereplication strategy for the identification of natural metabolites directly within mixtures. Exploiting the polarity range of metabolites, the principle was to rapidly fractionate a multigram quantity of a crude extract by centrifugal partition extraction (CPE). The obtained fractions of simplified chemical composition were subsequently analyzed by C-13 NMR After automatic collection and alignment of C-13 signals across spectra, hierarchical clustering analysis (HCA) was performed for pattern recognition. As a result, strong correlations between C-13 signals of a single structure within the mixtures of the fraction series were visualized as chemical shift clusters. Each cluster was finally assigned to a molecular structure with the help of a locally built C-13 NMR chemical shift database. The proof of principle of this strategy was achieved on a simple model mixture of commercially available plant secondary metabolites and then applied to a bark extract of the African tree Anogeissus leiocarpus Guill. & Perr. (Combretaceae). Starting from 5 g of this genuine extract, the fraction series was generated by CPE in only 95 min. C-13 NMR analyses of all fractions followed by pattern recognition of C-13 chemical shifts resulted in the unambiguous identification of seven major compounds, namely, sericoside, trachelosperogenin E, ellagic acid, an epimer mixture of (+)-gallocatechin and (-)-epigallocatechin, 3,3'-di-O-methylellagic acid 4'-O-xylopyranoside, and 3,4,3'-tri-O-methylflavellagic acid 4'-O-glucopyranoside.
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