期刊
ANALYTICAL CHEMISTRY
卷 86, 期 4, 页码 1980-1987出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac402742m
关键词
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资金
- National Natural Science Foundation of China [20905032, 21065005, 21103214, 21165010]
- Young Scientist Foundation of Jiangxi Province [20112BCB23006, 20122BCB23011]
- State Key Laboratory of Electroanalytical Chemistry [SKLEAC201310]
- Foundation of Jiangxi Educational Committee [GJJ13243, GJJ13244]
A facile and effective electrochemical sensing platform for the detection of glucose and urea in one sample without separation was developed using chitosan-reduced graphene oxide (CS-rGO)/concanavalin A (Con A) as a sensing layer. The CS-rGO/Con A with pH-dependent surface net charges exhibited pH-switchable response to negatively charged Fe(CN)(6)(3-). The principle for glucose and urea detection was essentially based on in situ pH-switchable enzyme-catalyzed reaction in which the oxidation of glucose catalyzed by glucose oxidase or the hydrolyzation of urea catalyzed by urease resulted in a pH change of electrolyte solution to give different electrochemical responses toward Fe(CN)(6)(3-). It was verified by cyclic voltammograms, differential pulse voltammograms, and electrochemical impedance spectroscopy. The resistance to charge transfer or amperometric current changed proportionally toward glucose concentration from 1.0 to 10.0 mM and urea concentration from 1.0 to 7.0 mM. On the basis of human serum experiments, the sensing platform was proved to be suitable for simultaneous assay of glucose and urea in a practical biosystem. This work not only gives a way to detect glucose and urea in one sample without separation but also provides a potential strategy for the detection of nonelectroactive species based on the enzyme-catalyzed reaction and pH-switchable biosensor.
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