期刊
ANALYTICAL CHEMISTRY
卷 86, 期 5, 页码 2380-2386出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac4040357
关键词
-
资金
- University of Illinois at Chicago
- UIC Chancellors Discovery Fund
- W. C. and May Preble Deiss Award for Graduate Research
- U.S. Air Force Surgeon General's Office of Modernization [FA7014-07-C-0047]
- American Chemical Society Petroleum Research Fund (ACS PRF) [50859-ND10]
We have created a platform for the ratiometric fluorescent sensing of targeted proteins by conjugating conjoined protein binding agent/organic dye ligands to water-soluble, emissive semiconductor quantum dots (QDs). The QD emission is tuned such that it may serve as an energy transfer donor to the dye acceptor. Upon exposure to the target proteins, these analytes bind to the surfaces of the QDs and change the microenvironments of the QD-bound dyes such that the emissive properties of the dyes are perturbed. The resulting alteration in the QD and dye fluorescence spectra creates a readout that is fully quantitative. The advantage of our methodology is that the detection of proteins is very fast as the platform is fully homogeneous, whereas the heterogeneous ELISA assay involves multiple steps with blocking agents and secondary reporters that ultimately complicate the process. The calculated detection limits for the two QD protein-sensing examples reported here are also competitive with the ubiquitous ELISA assay.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据