期刊
ANALYTICAL CHEMISTRY
卷 86, 期 14, 页码 6968-6975出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac500897t
关键词
-
资金
- Purdue University
- Agricultural Research Service of the United States Department of Agriculture [1935-42000-035]
- University of Illinois at Urbana-Champaign
- NIH [R01-CA20003]
- NSF I/IUCRC CABPN (Center for Agricultural, Biomedical and Pharmaceutical Nanotechnology) Grant at UIUC
- Taiwan Semiconductor Manufacturing Company [2012-06536]
Electrical detection of nucleic acid amplification through pH changes associated with nucleotide addition enables miniaturization, greater portability of testing apparatus, and reduced costs. However, current ion-sensitive field effect transistor methods for sensing nucleic acid amplification rely on establishing the fluid gate potential with a bulky, difficult to microfabricate reference electrode that limits the potential for massively parallel reaction detection. Here we demonstrate a novel method of utilizing a microfabricated solid-state quasi-reference electrode (QRE) paired with a pH-insensitive reference field effect transistor (REFET) for detection of real-time pH changes. The end result is a 0.18 mu m, silicon-on-insulator, foundry-fabricated sensor that utilizes a platinum QRE to establish a pH-sensitive fluid gate potential and a PVC membrane REFET to enable pH detection of loop mediated isothermal amplification (LAMP). This technique is highly amendable to commercial scale-up, reduces the packaging and fabrication requirements for ISFET pH detection, and enables massively parallel droplet interrogation for applications, such as monitoring reaction progression in digital PCR.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据