期刊
BIOSENSORS & BIOELECTRONICS
卷 22, 期 12, 页码 3283-3287出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2007.03.006
关键词
urea; activity; urease immobilization; urea biosensor; nanoporous alumina membranes
The urease was immobilized onto nanoporous alumina membranes prepared by the two-step anodization method, and a novel piezoelectric urea sensing system with separated porous alumina/urease electrode has been developed through measuring the conductivity change of immobilized urease/urea reaction. The process of urease immobilization was optimized and the performance of the developed urea biosensor was evaluated. The obtained urea biosensor presented high-selectivity monitoring of urea, better reproducibility (S.D. = 0.02, n = 6), shorter response time (30 s), wider linear range (0.5 mu M to 3 mM), lower detection limit (0.2 mu M) and good long-term storage stability (with about 76% of the enzymatic activity retained after 30 days). The clinical analysis of the urea biosensor confirmed the feasibility of urea detection in urine samples. (c) 2007 Elsevier B.V. All rights reserved.
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