期刊
ANALYTICAL CHEMISTRY
卷 86, 期 18, 页码 9013-9019出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac501418g
关键词
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资金
- Associazione Italiana per la Ricerca sul Cancro, AIRC [14420]
- European Research Council, ERC [336493]
- Int. Research Staff Exchange Scheme (IRSES) [269051]
- National Cancer Institute of the National Institute of Health [NRSA F31CA183385]
Here we investigate a novel signal-on electrochemical DNA sensor based on the use of a clamp-like DNA probe that binds a complementary target sequence through two distinct and sequential events, which lead to the formation of a triplex DNA structure. We demonstrate that this target-binding mechanism can improve both the affinity and specificity of recognition as opposed to classic probes solely based on Watson-Crick recognition. By using electrochemical signaling to report the conformational change, we demonstrate a signal-on E-DNA sensor with up to 400% signal gain upon target binding. Moreover, we were able to detect with nanomolar affinity a perfectly matched target as short as 10 bases (K-D = 0.39 nM). Finally, thanks to the molecular double-check provided by the concomitant Watson-Crick and Hoogsteen base pairings involved in target recognition, our sensor provides excellent discrimination efficiency toward a single-base mismatched target.
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