期刊
ANALYTICAL CHEMISTRY
卷 86, 期 9, 页码 4559-4565出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac500591n
关键词
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资金
- 973 Program [2012CB932600]
- National Natural Science Foundation [21025522, 21327902, 21305068, 21121091]
- Natural Science Foundation of Jiangsu Province [BK20130666]
- Postdoctoral Science Foundation of China [2013M540432]
This work describes a new electrochemiluminescence resonance energy transfer (ECL-RET) system with graphene oxide(GO)-Au/RuSi@Ru(bpy)(3)(2+)/chitosan (CS) composites as the ECL donor and Au@Ag2S nanoparticles (NPs) as ECL the acceptor for the first time. The ECL signal observed by the application of GO-Au/RuSi@Ru(bpy)(3)(2+)/CS composites was enhanced for 5-fold compared to that of RuSi@Ru(bpy)(3)(2+)/CS in the presence of coreactant tripropylamine (TPA) due to the increased surface area and improved electrical conductivity by using graphene oxide-gold nanoparticles (GO-Au) composite materials. In addition, we synthesized Au@Ag2S core-shell NPs, whose UV-vis absorption spectrum shows good spectral overlap with the ECL spectrum of GO-Au/RuSi@Ru(bpy)(3)(2+)/CS composites by adjusting the amount of Na2S and AgNO3 in the process of synthesis. The distance between energy donor and acceptor was studied to get the highly effective ECL-RET. Then, this ECL-RET system was developed for sensitive and specific detection of target DNA, and the ECL quenching efficiency (Delta I/I-0, Delta I = I-0 - I) was found to be logarithmically related to the concentration of the target DNA in the range from 10 aM to 10 pM.
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