Brucella suis urease encoded by ure1 but not ure2 is necessary for intestinal infection of BALB/c mice

Background: In prokaryotes, the ureases are multi-subunit, nickel-containing enzymes that catalyze the hydrolysis of urea to carbon dioxide and ammonia. The Brucella genomes contain two urease operons designated as ure1 and ure2. We investigated the role of the two Brucella suis urease operons on the infection, intracellular persistence, growth, and resistance to low-pH killing. Results: The deduced amino acid sequence of urease-a subunits of operons-1 and-2 exhibited substantial identity with the structural ureases of alpha-and beta-proteobacteria, Gram-positive and Gram-negative bacteria, fungi, and higher plants. Four ure deficient strains were generated by deleting one or more of the genes encoding urease subunits of B. suis strain 1330 by allelic exchange: strain 1330 Delta ureIK (generated by deleting ureD and ureA in ure1 operon), strain 1330 Delta ure2K (ureB and ureC in ure2 operon), strain 1330 Delta ure2C (ureA, ureB, and ureC in ure2 operon), and strain 1330 Delta ure1K Delta ure2C (ureD and ureA in ure1 operon and ureA, ureB, and ureC in ure2 operon). When grown in urease test broth, strains 1330, 1330 Delta ure2K and 1330 Delta ure2C displayed maximal urease enzyme activity within 24 hours, whereas, strains 1330 Delta ureIK and 1330 Delta ure1K Delta ure2C exhibited zero urease activity even 96 h after inoculation. Strains 1330 Delta ureIK and 1330 Delta ureIK Delta ure2C exhibited slower growth rates in tryptic soy broth relative to the wild type strain 1330. When the BALB/ c mice were infected intraperitoneally with the strains, six weeks after inoculation, the splenic recovery of the ure deficient strains did not differ from the wild type. In contrast, when the mice were inoculated by gavage, one week after inoculation, strain 1330 Delta ure1K Delta ure2C was cleared from livers and spleens while the wild type strain 1330 was still present. All B. suis strains were killed when they were incubated in-vitro at pH 2.0. When the strains were incubated at pH 2.0 supplemented with 10 mM urea, strain 1330 Delta ureIK was completely killed, strain 1330 Delta ure2C was partially killed, but strains 1330 and 1330 Delta ure2K were not killed. Conclusion: These findings suggest that the ure1 operon is necessary for optimal growth in culture, urease activity, resistance against low-pH killing, and in vivo persistence of B. suis when inoculated by gavage. The ure2 operon apparently enhances the resistance to low-pH killing in-vitro.