期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1154, 期 1-2, 页码 473-476出版社
ELSEVIER
DOI: 10.1016/j.chroma.2007.04.022
关键词
microwave heating; poly-3-hydroxybutyrate; esterification; methyl 3-hydroxybutyrate; Alcaligenes latus
In the present study, we used microwave energy instead of conventional heating to transform poly-3-hydroxybutyrate (PHB) into methyl 3-hydroxybutyrate (Me-3HB) in acidified methanol (H2SO4, 10%, v/v) mixture in less than 4 min at 10% microwave power. The microwave assisted method was then applied to analyze PHB produced by Alcaligenes latus. The PHB content in the biomass determined using microwave heating was comparable to the amount found by conventional heating. Moreover, the new esterification method was at least 50 times faster than the conventional method, affording a significant saving of time and energy. Crown Copyright (c) 2007 Published by Elsevier B.V. All rights reserved.
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